MUTANOLYSIN

RECOMBINANT

Recombinant mutanolysin

A&A Biotechnology is proud to offer recombinant mutanolysin expressed in bacteria. We are first to produce recombinant version of this enzyme worldwide. Our recombinant mutanolysin exhibits superior purity and affordable price, compared to native alternative products available from other vendors. Enzyme is safe, being a product originating from organism recognized by U.S Food and Drug Administration as GRAS (Generally Recognized as Safe).

General description

Mutanolysin (EC 3.2.1.17) (N-acetylmuramidase) is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall structures abundant in many gram-positive bacteria.

Application

Recombinant mutanolysin effectively digests cell wall of particulary resistant to lysis bacteria.

Including but not limited to: Streptococcus, Lactobacillus, Lactococcus and Listeria.

mutanolysin sds-page

Concentration

10 U/µl


Unit definition

One unit will produce a ∆A600 nm of 0.01 per minute at pH 6.0 at 37 °C in a 1 ml volume using suspension of Streptococcus faecalis cell wall as substrate.


Products

Mutanolysin (recombinant), 5 000 U, cat. # 1017-5

Mutanolysin (recombinant), 10 000 U, cat. # 1017-10

Mutanolysin (recombinant), 50 000 U, cat. # 1017-50

Bulk quantities available upon request. Contact us at: info@aabiot.com

Mutanolysin is also available as a part of our dedicated kits for genomic DNA isolation:
Genomic Mini AX Bacteria+ SPIN, cat. # 060-100MS
Genomic Mini AX Bacteria+ GRAVITY, cat. # 102-100M
Genomic Mini AX Bacteria+, cat. #060-60M


Ordering

free samples available!

orders@aabiot.com, +48 58 6982194

visit us at www.aabiot.com

Recombinant mutanolysin and lysozyme synergism

Mutanolysin lytic activity may differ substantially when lysing various, even closely related, bacteria. The lysis effectiveness depends on peptidoglycan cell wall structure and bacteria growth conditions. It is especially important issue to be considered when processing environmental samples. For some hard-to-lyse microorganisms the application of mutanolysin and lysozyme mixture may drastically increase the DNA/RNA yield. Please see below the synergistic effect of lytic activities of recombinant mutanolysin and lysozyme mixture.
For supplementing lysozyme application we recommend Lysozyme (cat. # 1005-10 or 1005-50).

mutanolysin and lysozyme synergism