Recombinant mutanolysin
A&A Biotechnology is proud to offer recombinant mutanolysin expressed in bacteria. We are first to produce recombinant version of this enzyme worldwide. Our recombinant mutanolysin exhibits superior purity and affordable price, compared to native alternative products available from other vendors. Enzyme is safe, being a product originating from an organism recognized by U.S Food and Drug Administration as GRAS (Generally Recognized as Safe).
General description
Mutanolysin (EC 3.2.1.17) (N-acetylmuramidase) is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine (NAM-NAG) linkage of bacterial cell wall peptidoglycan. Its carboxy terminal moieties are involved in recognition and binding of unique cell wall structures abundant in many gram-positive bacteria.
Application
- Enzymatic cell lysis in DNA and RNA isolation processes
- Enzymatic cell lysis for diagnostics
- Mild formation of spheroplasts of gram-positive bacteria
- Effective lysis of gram-positive bacteria in environmental studies and DNA-based microbial detection
Recombinant mutanolysin & lysozyme synergism
Mutanolysin lytic activity may differ substantially when lysing various, even closely related, bacteria. The lysis effectiveness depends on peptidoglycan cell wall structure and bacteria growth conditions. It is an especially important issue to be considered when processing environmental samples. For some hard-to-lyse microorganisms the application of mutanolysin and lysozyme mixture may drastically increase the DNA/RNA yield. Please see below the synergistic effect of lytic activities of recombinant mutanolysin and lysozyme mixture. For supplementing lysozyme application we recommend Lysozyme (cat. # 1005-10 or 1005-50).
Specification
| Origin | Recombinant, GRAS status host |
|---|---|
| Unit definition | One unit per mL produces ΔA600 = 0.01/min at pH 6.0, 37°C using S. faecalis cell walls. |
| Formulation | Solution or lyophilisate |
| Specific activity | > 10,000 U/mg |
| Purification | Always multistep liquid chromatography |
| Expiry | 3 years |
| Stability | Extremely stable, even at room temperature |
| DNA/DNase/RNase | DNA-free, DNase-free, RNase-free |
| Packaging | Standard or custom, OEM (direct producer) |
| Shipment | Lyophilisate: room temperature; Solution: reusable cold gel packs |
Ordering info
Recombinant Mutanolysin is offered as a solution or lyophilised. Bulk quantities and OEM is available.
Mutanolysin (solution) Mutanolysin (lyophilisate)
Bulk quantities available upon request. Contact us at: info@aabiot.com.
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